Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion

Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana 

Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.

The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.

Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.

The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.

 

KEY POINTS:

• The TBSV-based transient expression system has been significantly improved.

• The necrotic effects caused by viral P19 protein were avoided by the usage of N.   excelsiana as a host plant.

• The expression level of the non-fusion vector was similar to the most effective virus   vectors reported so far.

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-48Tests	GenAsia Biotech	48 Tests	Ask for price

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-96T	GenAsia Biotech	96T	EUR 559.2

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-96Tests	GenAsia Biotech	96 Tests	EUR 388

human CD163 antigen,CD163 LISA kit
201-12-0247	Shanghai Sunred Biological Technology	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human CD163 antigen,CD163 ELISA KIT
E0246Hu-1096T	Jiaxing Korain Biotech Ltd (BT Labs)	10*96T	EUR 4122

Human CD163 antigen,CD163 ELISA KIT
E0246Hu-48wells	Jiaxing Korain Biotech Ltd (BT Labs)	48 wells	EUR 300

Human CD163 antigen,CD163 ELISA KIT
E0246Hu-596T	Jiaxing Korain Biotech Ltd (BT Labs)	5*96T	EUR 2061

Human CD163 antigen,CD163 ELISA KIT
E0246Hu-96wells	Jiaxing Korain Biotech Ltd (BT Labs)	96 wells	EUR 458

Human CD163 antigen,CD163 ELISA KIT
JOT-EK0246Hu	Jotbody	96 wells	Ask for price
Description: Human

Human CD163 antigen, CD163 ELISA Kit
MBS165165-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3460

Human CD163 antigen, CD163 ELISA Kit
MBS165165-48StripWells	MyBiosource	48-Strip-Wells	EUR 285

Human CD163 antigen, CD163 ELISA Kit
MBS165165-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1750

Human CD163 antigen, CD163 ELISA Kit
MBS165165-96StripWells	MyBiosource	96-Strip-Wells	EUR 425

Human CD163 Antigen (CD163) ELISA Kit
MBS9353075-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6725

Human CD163 Antigen (CD163) ELISA Kit
MBS9353075-48StripWells	MyBiosource	48-Strip-Wells	EUR 550

Human CD163 Antigen (CD163) ELISA Kit
MBS9353075-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3420

Human CD163 Antigen (CD163) ELISA Kit
MBS9353075-96StripWells	MyBiosource	96-Strip-Wells	EUR 765

human CD163 antigen,CD163 ELISA kit
YLA0098HU-48T	Shanghai YL Biotech	48T	Ask for price

human CD163 antigen,CD163 ELISA kit
YLA0098HU-96T	Shanghai YL Biotech	96T	Ask for price

Human CD163 Antigen, CD163 GENLISA ELISA
KBH0246	Krishgen	1 x 96 wells	EUR 286

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47162MH	Creative Diagnostics	25 µg	EUR 322.14
Description: Mouse

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47163MH	Creative Diagnostics	0.2 mg	EUR 659.4
Description: Mouse

Human CD163 antigen-CD163-ISA kit
QY-E05066	Qayee Biotechnology	96T	EUR 400

Mouse Anti Human CD163, APC Conjugated mAb
MBS9431893-100Tests	MyBiosource	100Tests	EUR 520

Mouse Anti Human CD163, APC Conjugated mAb
MBS9431893-25Tests	MyBiosource	25Tests	EUR 275

Mouse Anti Human CD163, APC Conjugated mAb
MBS9431893-5x100Tests	MyBiosource	5x100Tests	EUR 2195

CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 2
SC308167	Origene Technologies GmbH	10 µg	Ask for price

CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 1
SC117495	Origene Technologies GmbH	10 µg	Ask for price

PE anti-human CD163
E16FHP163-025	EnoGene	25 tests	EUR 433.33
Description: Available in various conjugation types.

PE anti-human CD163
E16FHP163-100	EnoGene	100 tests	EUR 953.33
Description: Available in various conjugation types.

Anti-Human CD163 PE
MBS696987-100Tests	MyBiosource	100Tests	EUR 415

Anti-Human CD163 PE
MBS696987-25Tests	MyBiosource	25Tests	EUR 225

Anti-Human CD163 PE
MBS696987-5x100Tests	MyBiosource	5x100Tests	EUR 1820

Anti-Human CD163 Antibody
101-M291	ReliaTech	100 µg	EUR 399
Description: CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes.

APC anti-human CD163
E16FHA163-025	EnoGene	25 tests	EUR 520
Description: Available in various conjugation types.

APC anti-human CD163
E16FHA163-100	EnoGene	100 tests	EUR 1191.67
Description: Available in various conjugation types.

Anti-Human CD163 APC
MBS696989-100Tests	MyBiosource	100Tests	EUR 480

Anti-Human CD163 APC
MBS696989-25Tests	MyBiosource	25Tests	EUR 245

Anti-Human CD163 APC
MBS696989-5x100Tests	MyBiosource	5x100Tests	EUR 2115

APC anti-human CD163
MBS9459445-100Tests	MyBiosource	100Tests	EUR 655

APC anti-human CD163
MBS9459445-25Tests	MyBiosource	25Tests	EUR 355

APC anti-human CD163
MBS9459445-5x100Tests	MyBiosource	5x100Tests	EUR 2790

FITC anti-human CD163
E16FHF163-025	EnoGene	25 tests	EUR 520
Description: Available in various conjugation types.

FITC anti-human CD163
E16FHF163-100	EnoGene	100 tests	EUR 1191.67
Description: Available in various conjugation types.

Biotin anti-human CD163
E16FHB163-025U	EnoGene	25 μg	EUR 346.67
Description: Available in various conjugation types.

Biotin anti-human CD163
E16FHB163-100U	EnoGene	100 μg	EUR 780
Description: Available in various conjugation types.

Purified anti-human CD163
E16FHU163-100U	EnoGene	100 μg	EUR 433.33
Description: Available in various conjugation types.

CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 1
RG208557	Origene Technologies GmbH	10 µg	Ask for price

CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 2
RG212754	Origene Technologies GmbH	10 µg	Ask for price

Rabbit Anti Human CD163 Monoclonal Clone ABCC-3
IRBAHUCD163ABCC3C100UL	Innovative research	each	EUR 496
Description: Rabbit Anti Human CD163 Monoclonal Clone ABCC-3

Recombinant Human CD163/CD163 Protein, His Tag
E40KMH346	EnoGene	20ug	EUR 495

Human CD163 siRNA
20-abx910931	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 2
RC212754	Origene Technologies GmbH	10 µg	Ask for price

CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1
RC208557	Origene Technologies GmbH	10 µg	Ask for price

Human CD163 Protein
E40KMPH6242	EnoGene	20ug	EUR 495

Recombinant Human CD163
MBS147285-0001mg	MyBiosource	0.001mg	EUR 240

Recombinant Human CD163
MBS147285-0005mg	MyBiosource	0.005mg	EUR 310

Recombinant Human CD163
MBS147285-005mg	MyBiosource	0.05mg	EUR 1360

Recombinant Human CD163
MBS147285-5x005mg	MyBiosource	5x0.05mg	EUR 5800

CD163 siRNA (Human)
MBS8229683-15nmol	MyBiosource	15nmol	EUR 405

CD163 siRNA (Human)
MBS8229683-30nmol	MyBiosource	30nmol	EUR 565

CD163 siRNA (Human)
MBS8229683-5x30nmol	MyBiosource	5x30nmol	EUR 2450

Anti-Human CD163 Antibody (M130)
MBS1564482-01mg	MyBiosource	0.1mg	EUR 340

Anti-Human CD163 Antibody (M130)
MBS1564482-5x01mg	MyBiosource	5x0.1mg	EUR 1235

Anti-Human CD163 Antibody (AB101)
MBS1570008-01mg	MyBiosource	0.1mg	EUR 405

Anti-Human CD163 Antibody (AB101)
MBS1570008-5x01mg	MyBiosource	5x0.1mg	EUR 1520

PE-Cy7 anti-human CD163
E16FHN163-025	EnoGene	25 tests	EUR 650
Description: Available in various conjugation types.

PE-Cy7 anti-human CD163
E16FHN163-100	EnoGene	100 tests	EUR 1408.33
Description: Available in various conjugation types.

APC-Cy7 anti-human CD163
E16FHO163-025	EnoGene	25 tests	EUR 650
Description: Available in various conjugation types.

APC-Cy7 anti-human CD163
E16FHO163-100	EnoGene	100 tests	EUR 1408.33
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-human CD163
E16FHS163-025	EnoGene	25 tests	EUR 541.67
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-human CD163
E16FHS163-100	EnoGene	100 tests	EUR 1278.33
Description: Available in various conjugation types.

Anti-Human CD163 PE-Cyanine7
MBS696988-100Tests	MyBiosource	100Tests	EUR 505

Anti-Human CD163 PE-Cyanine7
MBS696988-25Tests	MyBiosource	25Tests	EUR 270

Anti-Human CD163 PE-Cyanine7
MBS696988-5x100Tests	MyBiosource	5x100Tests	EUR 2220

Mouse Monoclonal anti-human CD163
hAP-0161	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human CD163
hAP-0161A	Angio Proteomie	50ug	EUR 400

Human CD163, Fc Tag
E24PHA282	EnoGene	50 μg	EUR 250

Human, Rat, Mouse CD163 antibody
P0405	Omnimabs	100ul	EUR 150

Human CD163, His Tag
E24PHA281	EnoGene	50 μg	EUR 250

Human CD163 ELISA Kit
ELA-E1726h	Lifescience Market	96 Tests	EUR 988.8

Human CD163 ELISA Kit
EK5296	SAB	96 tests	EUR 599

Human CD163 ELISA Kit
GWB-SKR207	GenWay Biotech	96 Tests	Ask for price

Human CD163 ELISA Kit
LF-EK50890	Abfrontier	1×96T	EUR 777.6

Human CD163 ELISA Kit
MBS3800092-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6725

Human CD163 ELISA Kit
MBS3800092-48StripWells	MyBiosource	48-Strip-Wells	EUR 550

Human CD163 ELISA Kit
MBS3800092-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3420

Human CD163 ELISA Kit
MBS3800092-96StripWells	MyBiosource	96-Strip-Wells	EUR 765

Human CD163 ELISA Kit
MBS824474-5x96Tests	MyBiosource	5x96Tests	EUR 2880

Human CD163 ELISA Kit
MBS824474-96Tests	MyBiosource	96Tests	EUR 645

Human CD163 shRNA Plasmid
20-abx956176	Abbexa	Ask for price Ask for price	150 µg 300 µg

Human CD163 Protein Lysate
MBS138999-002mg	MyBiosource	0.02mg	EUR 365

Human CD163 Protein Lysate
MBS138999-5x002mg	MyBiosource	5x0.02mg	EUR 1410

Human CD163 Recombinant Protein
PROTQ86VB7	BosterBio	10ug	EUR 350
Description: Human CD163 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 42-1050aa. Application(s): SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

Recombinant Human CD163 Protein
RP02295	Abclonal	500μg	EUR 244.43

CD163 (Human) ELISA Kit
EKA52023-5x96T	Biomatik Corporation	5x96T	Ask for price

CD163 (Human) ELISA Kit
EKA52023-96T	Biomatik Corporation	96T	EUR 446.9

Human CellExp? CD163, Human recombinant
9226-10	Biovision	each	EUR 366

Human CellExp? CD163, Human recombinant
9226-50	Biovision	each	EUR 927.6

anti- CD163/M130 Antibody
FNab09771	FN Test	100µg	EUR 658.5
Description: Antibody raised against CD163/M130

Mouse Monoclonal anti-Human CD163 Antibody
xAP-0134	Angio Proteomie	100ug	EUR 280

Mouse Monoclonal anti-Human CD163 Antibody
xAP-0135	Angio Proteomie	100ug	EUR 280

CD163 Rabbit anti-Human Polyclonal Antibody
MBS2402782-005mL	MyBiosource	0.05mL	EUR 615

CD163 Rabbit anti-Human Polyclonal Antibody
MBS2402782-5x005mL	MyBiosource	5x0.05mL	EUR 2605

CD163 Rabbit anti-Human Polyclonal Antibody
MBS2403148-005mg	MyBiosource	0.05mg	EUR 615

CD163 Rabbit anti-Human Polyclonal Antibody
MBS2403148-5x005mg	MyBiosource	5x0.05mg	EUR 2605

Anti-Hu CD163 PE
1P-645-T025	ExBio	25 tests	EUR 121

Anti-Hu CD163 PE
1P-645-T100	ExBio	100 tests	EUR 242

Anti-Hu CD163 PE
MBS1800644-100Tests	MyBiosource	100Tests	EUR 345

Anti-Hu CD163 PE
MBS1800644-5x100Tests	MyBiosource	5x100Tests	EUR 1300

Anti-Hu CD163 APC
1A-645-T025	ExBio	25 tests	EUR 121

Anti-Hu CD163 APC
1A-645-T100	ExBio	100 tests	EUR 242

Anti-Hu CD163 APC
MBS1800643-100Tests	MyBiosource	100Tests	EUR 345

Anti-Hu CD163 APC
MBS1800643-5x100Tests	MyBiosource	5x100Tests	EUR 1300

MOUSE ANTI PIG CD163
MBS224621-01mg	MyBiosource	0.1mg	EUR 420

MOUSE ANTI PIG CD163
MBS224621-5x01mg	MyBiosource	5x0.1mg	EUR 1715

MOUSE ANTI RAT CD163
MBS216354-01mg	MyBiosource	0.1mg	EUR 455

MOUSE ANTI RAT CD163
MBS216354-5x01mg	MyBiosource	5x0.1mg	EUR 1885

MOUSE ANTI RAT CD163
MBS216356-025mg	MyBiosource	0.25mg	EUR 620

MOUSE ANTI RAT CD163
MBS216356-5x025mg	MyBiosource	5x0.25mg	EUR 2620

Anti-Hu CD163 Purified
11-645-C025	ExBio	0.025 mg	EUR 71.5

Anti-Hu CD163 Purified
11-645-C100	ExBio	0.1 mg	EUR 143

Anti-Hu CD163 Purified
MBS1800642-01mg	MyBiosource	0.1mg	EUR 260

Anti-Hu CD163 Purified
MBS1800642-5x01mg	MyBiosource	5x0.1mg	EUR 930

Human CD163 Protein, His Tag
CD3-H52E8	ACROBIOSYSTEMS	1mg	EUR 381.5
Description: Human CD163, His Tag (CD3-H52E8) is expressed from human 293 cells (HEK293). It contains AA Ser 42 - Ser 1045 (Accession # Q86VB7).

Anti-Human CD163 (ABT-CD163) Mouse Monoclonal Antibody (Ready to Use)
MBS9511396-12mLRTU	MyBiosource	12mL(RTU)	EUR 530

Anti-Human CD163 (ABT-CD163) Mouse Monoclonal Antibody (Ready to Use)
MBS9511396-3mLRTU	MyBiosource	3mL(RTU)	EUR 240

Anti-Human CD163 (ABT-CD163) Mouse Monoclonal Antibody (Ready to Use)
MBS9511396-5x12mLRTU	MyBiosource	5x12mL(RTU)	EUR 2230

Anti-Human CD163 (ABT-CD163) Mouse Monoclonal Antibody (Ready to Use)
MBS9511396-6mLRTU	MyBiosource	6mL(RTU)	EUR 325

CD163 Antigen (CD163) Rabbit anti-Human Polyclonal (N-Terminus) (K20-T) Antibody
GWB-B1E200	GenWay Biotech	0.05 ml	Ask for price

Human CD163 knockout cell line
ABC-KH2671	AcceGen	1 vial	Ask for price
Description: Human CD163 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CD163 PicoKine ELISA Kit
MBS176496-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2755

Human CD163 PicoKine ELISA Kit
MBS176496-96StripWells	MyBiosource	96-Strip-Wells	EUR 600

Human CD163 knockdown cell line
ABC-KD2671	AcceGen	1 vial	Ask for price
Description: Human CD163 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CD163 Protein Lysate 20ug
IHUCD163PLLY20UG	Innovative research	each	EUR 213
Description: Human CD163 Protein Lysate 20ug

Rabbit Polyclonal to Human CD163
MBS245302-0125mL	MyBiosource	0.125mL	EUR 620

Rabbit Polyclonal to Human CD163
MBS245302-5x0125mL	MyBiosource	5x0.125mL	EUR 2695

CD163 (NM_004244) Human Recombinant Protein
E45H38041M5	EnoGene	20 ug	EUR 785.42

CD163, Human, mAb RM3/1
MBS584200-01mg	MyBiosource	0.1mg	EUR 710

CD163, Human, mAb RM3/1
MBS584200-05mg	MyBiosource	0.5mg	EUR 1695

CD163, Human, mAb RM3/1
MBS584200-1mg	MyBiosource	1mg	EUR 2370

CD163, Human, mAb RM3/1
MBS584200-5mg	MyBiosource	5mg	EUR 7845

Recombinant Human CD163, GST-tagged
CD163-10910H	Creative BioMart	25ug	EUR 255.2
Description: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.

CD163 ORF Vector (Human) (pORF)
ORF017045	ABM	1.0 ug DNA	EUR 486

CD163 (Human) LumiAb ELISA Kit
MBS9510961-1x96Wells	MyBiosource	1x96Wells	EUR 520

CD163 (Human) LumiAb ELISA Kit
MBS9510961-5x96Wells	MyBiosource	5x96Wells	EUR 2395

PE Anti-human CD163 Antibody *GHI/61*
116301K0	AAT Bioquest	25 tests	EUR 391
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*
116301K0-25tests	AAT Bioquest	25 tests	EUR 383
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*
116301K1	AAT Bioquest	100 tests	EUR 1015
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*
116301K1-100tests	AAT Bioquest	100 tests	EUR 995
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*
116301K2	AAT Bioquest	500 tests	EUR 3044
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*
116301K2-500tests	AAT Bioquest	500 tests	EUR 2984
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

CD163 (Human) OmniKine ELISA Kit
MBS9502033-1x96Wells	MyBiosource	1x96Wells	EUR 500

CD163 (Human) OmniKine ELISA Kit
MBS9502033-5x96Wells	MyBiosource	5x96Wells	EUR 2300

CD163 (Human) Sandwich ELISA Kit
OK-0312	Assay Biotech	1Kit	EUR 280
Description: CD163 (Human) Colorimetric Sandwich ELISA Kit

APC Anti-human CD163 Antibody *GHI/61*
116301B0	AAT Bioquest	25 tests	EUR 391
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*
116301B0-25tests	AAT Bioquest	25 tests	EUR 383
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*
116301B1	AAT Bioquest	100 tests	EUR 1015
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*
116301B1-100tests	AAT Bioquest	100 tests	EUR 995
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*
116301B2	AAT Bioquest	500 tests	EUR 3044
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*
116301B2-500tests	AAT Bioquest	500 tests	EUR 2984
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Alexa Fluor® 647 anti-human CD163
E16FHK163-025	EnoGene	25 tests	EUR 433.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD163
E16FHK163-100	EnoGene	100 tests	EUR 975
Description: Available in various conjugation types.

CD163 ELISA Kit (Human) (OKCD07621)
OKCD07621	Aviva Systems Biology	96 Wells	EUR 1123.2
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL

CD163 ELISA Kit (Human) (OKBB00786)
OKBB00786	Aviva Systems Biology	96 Wells	EUR 606
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

CD163 ELISA Kit (Human) (OKBB01513)
OKBB01513	Aviva Systems Biology	96 Wells	EUR 684
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

CD163 ELISA Kit (Human) (OKAN04666)
OKAN04666	Aviva Systems Biology	96 Wells	EUR 950.4
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL

FITC Anti-human CD163 Antibody *GHI/61*
116301G0	AAT Bioquest	100 tests	EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

FITC Anti-human CD163 Antibody *GHI/61*
116301G0-100tests	AAT Bioquest	100 tests	EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

FITC Anti-human CD163 Antibody *GHI/61*
116301G1	AAT Bioquest	500 tests	EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

FITC Anti-human CD163 Antibody *GHI/61*
116301G1-500tests	AAT Bioquest	500 tests	EUR 2051
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

TRITC Anti-human CD163 Antibody *GHI/61*
116301H0	AAT Bioquest	100 tests	EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

TRITC Anti-human CD163 Antibody *GHI/61*
116301H0-100tests	AAT Bioquest	100 tests	EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

TRITC Anti-human CD163 Antibody *GHI/61*
116301H1	AAT Bioquest	500 tests	EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

TRITC Anti-human CD163 Antibody *GHI/61*
116301H1-500tests	AAT Bioquest	500 tests	EUR 2051
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S0	AAT Bioquest	25 tests	EUR 391
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S0-25tests	AAT Bioquest	25 tests	EUR 383
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S1	AAT Bioquest	100 tests	EUR 1015
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S1-100tests	AAT Bioquest	100 tests	EUR 995
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S2	AAT Bioquest	500 tests	EUR 3044
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*
116301S2-500tests	AAT Bioquest	500 tests	EUR 2984
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

QuickDetect? CD163 (Human) ELISA Kit
K4422-100	Biovision	each	EUR 966

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I0	AAT Bioquest	100 tests	EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I0-100tests	AAT Bioquest	100 tests	EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I1	AAT Bioquest	500 tests	EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I1-500tests	AAT Bioquest	500 tests	EUR 2051
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Purified Anti-human CD163 Antibody *GHI/61*
11630000	AAT Bioquest	100 ug	EUR 373
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Purified Anti-human CD163 Antibody *GHI/61*
11630000-100ug	AAT Bioquest	100 ug	EUR 365
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*
116301J0	AAT Bioquest	100 tests	EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*
116301J0-100tests	AAT Bioquest	100 tests	EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors

Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.

Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.

Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.

A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation

 

Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.

Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.

When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.

IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.

How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.