Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion

Clem May 20, 2021 0 Comments
grdr

Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana 

Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.

The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.

Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.

The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.

 

KEY POINTS:

• The TBSV-based transient expression system has been significantly improved.

  • The necrotic effects caused by viral P19 protein were avoided by the usage of N.   excelsiana as a host plant.
  • The expression level of the non-fusion vector was similar to the most effective virus   vectors reported so far.
grdr

grdr

Anti-CD163
DB-045-RTU-15 15 ml
EUR 354
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB-045-RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB045RTU-15 15 ml
EUR 354
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB045RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Mouse Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Mu-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids.

Mouse Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Mu-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids.

Rat Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Mu-48Tests 48 Tests
EUR 511

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Mu-96Tests 96 Tests
EUR 709

Rat Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Ra-48Tests 48 Tests
EUR 511

Rat Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Ra-96Tests 96 Tests
EUR 709

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Mu-48Tests 48 Tests
EUR 534

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Mu-96Tests 96 Tests
EUR 742

Rat Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Ra-48Tests 48 Tests
EUR 534

Rat Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Ra-96Tests 96 Tests
EUR 742

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349

Anti-CD163 Antibody
A00812-1 100ug/vial
EUR 334

Anti-CD163 antibody
STJ160082 1 mL C
EUR 941

Anti-CD163 antibody
STJ16100302 100 µg
EUR 720

Anti-CD163 antibody
STJ16100324 1 mL
EUR 979

Anti-CD163 antibody
STJ180301 0.1 ml
EUR 215

Anti-CD163 antibody
STJ190009 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1)

Anti-CD163 Antibody
STJ500423 100 µg
EUR 476

Anti-CD163 Antibody
STJ500424 100 µg
EUR 476

Anti-CD163 antibody
STJ110681 100 µl
EUR 277
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Anti-CD163 Antibody
PA1874 100ug/vial
EUR 294

Anti-CD163 Antibody
PA1874-1 100ug/vial
EUR 334

Anti-CD163/M130 antibody
PAab09771 100 ug
EUR 355

Anti-CD163/M130 Antibody
A00812 100ul
EUR 397
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details.

Anti-Hu CD163 Purified
11-645-C025 0.025 mg
EUR 99

Anti-Hu CD163 Purified
11-645-C100 0.1 mg
EUR 158

Anti-Hu CD163 APC
1A-645-T025 25 tests
EUR 140

Anti-Hu CD163 APC
1A-645-T100 100 tests
EUR 240

Anti-Hu CD163 PE
1P-645-T025 25 tests
EUR 140

Anti-Hu CD163 PE
1P-645-T100 100 tests
EUR 240

Anti-CD163 Antibody BIOTIN
STJ500425 100 µg
EUR 586

Anti-CD163 Antibody FITC
STJ500426 100 µg
EUR 586

Anti-CD163 Antibody BIOTIN
STJ500427 100 µg
EUR 586

Anti-CD163 Antibody FITC
STJ500428 100 µg
EUR 586

anti- CD163/M130 Antibody
FNab09771 100µg
EUR 548.75
Description: Antibody raised against CD163/M130 

human CD163 antigen,CD163 LISA kit
201-12-0247 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-48T 48T
EUR 289

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-96T 96T
EUR 466

Human CD163 antigen(CD163)ISA kit
QY-E05066 96T
EUR 361

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47162MH 25 µg
EUR 372

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47163MH 0.2 mg
EUR 881

Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280

Anti-Hu CD163 PE-Cy7
T7-645-T025 25 tests
EUR 154

Anti-Hu CD163 PE-Cy7
T7-645-T100 100 tests
EUR 268

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T025 25 tests
EUR 196

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T100 100 tests
EUR 352

Anti-CD163 Rabbit Monoclonal Antibody
M00812 100ug/vial
EUR 397
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human.

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-48T 48T
EUR 364

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-96T 96T
EUR 590

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-48T 48T
EUR 402

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-96T 96T
EUR 684

CD163 Antibody
ABD8235 100 ug
EUR 438

CD163 antibody
10R-CD163aRT 250 ug
EUR 716
Description: Mouse monoclonal CD163 antibody

CD163 antibody
10R-6496 100 ug
EUR 203
Description: Mouse monoclonal CD163 antibody

CD163 Antibody
DF8235 200ul
EUR 304
Description: CD163 Antibody detects endogenous levels of total CD163.

CD163 Antibody
1-CSB-PA969205
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

CD163 Antibody
1-CSB-PA801238LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

Cd163 Antibody
1-CSB-PA650452LA01MO
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

CD163 siRNA
20-abx910930
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CD163 siRNA
20-abx910931
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CD163 Antibody
20-abx242956
  • EUR 467.00
  • EUR 328.00
  • 100 ul
  • 50 ul

CD163 Antibody
37157-100ul 100ul
EUR 252

CD163 Antibody
49553-100ul 100ul
EUR 333

CD163 Antibody
49553-50ul 50ul
EUR 239

Human CD163 ELISA Kit
ELA-E1726h 96 Tests
EUR 824

Human CD163 ELISA Kit
LF-EK50890 1×96T
EUR 648

Human CD163 shRNA Plasmid
20-abx956176
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse Anti Rat Cd163 Monoclonal Antibody
DMABT-47170MR 0.25 mg
EUR 741

Mouse Anti Rat Cd163 Monoclonal Antibody
DMABT-47171MR 0.1 mg
EUR 559

Anti-Human CD163 DyLight® 488 conjugated Antibody
A00812-Dyl488 100ug/vial
EUR 344

Anti-Human CD163 DyLight® 550 conjugated Antibody
A00812-Dyl550 100ug/vial
EUR 344

Mouse Anti-Human CD163 monoclonal antibody, clone JID274
CABT-L2938-100uL500uL 100 uL, 500 uL
EUR 502

Human CellExp? CD163, Human recombinant
9226-10
EUR 305

Human CellExp? CD163, Human recombinant
9226-50
EUR 773

Mouse Anti Pig Cd163 Monoclonal Antibody,FITC
CABT-47164MP 0.1 mg
EUR 881

Mouse Anti Pig Cd163 Monoclonal Antibody,RPE
CABT-47166MP 100 TEST
EUR 715

Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
M00812-1 100uL
EUR 385
Description: Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 tested in WB, IHC, reactive to Human

Mouse Anti Rat Cd163 Monoclonal Antibody,Biotin
DMABT-47168MR 0.1 mg
EUR 881

Mouse Anti Rat Cd163 Monoclonal Antibody,FITC
DMABT-47169MR 0.1 mg
EUR 881

Mouse Anti Rat Cd163 Monoclonal Antibody,RPE
DMABT-47172MR 100 TEST
EUR 892

CD163 antibody (PE)
61R-1319 100 tests
EUR 403
Description: Mouse monoclonal CD163 antibody (PE)

CD163 antibody (biotin)
61R-1569 100 ug
EUR 349
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 antibody (biotin)
61R-CD163BHUBT 100 ug
EUR 921
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 antibody (biotin)
61R-CD163CHUBT 250 ug
EUR 781
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 Blocking Peptide
DF8235-BP 1mg
EUR 195

Porcine CD163 Protein
20-abx260472
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

Recombinant Porcine CD163
7-04750 10µg Ask for price

Recombinant Porcine CD163
7-04751 50µg Ask for price

Recombinant Porcine CD163
7-04752 1mg Ask for price

Cd163 Polyclonal Antibody
A53832 100 µg
EUR 570.55
Description: Ask the seller for details

CD163 Rabbit pAb
A8383-100ul 100 ul
EUR 308

CD163 Rabbit pAb
A8383-200ul 200 ul
EUR 459

CD163 Rabbit pAb
A8383-20ul 20 ul
EUR 183

CD163 Rabbit pAb
A8383-50ul 50 ul
EUR 223

CD163 Conjugated Antibody
C49553 100ul
EUR 397

CD163 Conjugated Antibody
C37157 100ul
EUR 397

CD163 ORF Vector (Human) (pORF)
ORF017045 1.0 ug DNA
EUR 405

QuickDetect? CD163 (Human) ELISA Kit
K4422-100
EUR 805

Human CD163 PicoKine ELISA Kit
EK0715 96 wells
EUR 425
Description: For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin).

ELISA kit for Human CD163
EK5296 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human CD163 in samples from serum, plasma, tissue homogenates and other biological fluids.

CD163 ELISA Kit (Human) (OKCD07621)
OKCD07621 96 Wells
EUR 936
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL

CD163 ELISA Kit (Human) (OKAN04666)
OKAN04666 96 Wells
EUR 792
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL

CD163 ELISA Kit (Human) (OKBB00786)
OKBB00786 96 Wells
EUR 505
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

CD163 ELISA Kit (Human) (OKBB01513)
OKBB01513 96 Wells
EUR 570
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

Human soluble CD163(sCD163) ELISA Kit
CSB-E14050h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163 (sCD163) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human soluble CD163(sCD163) ELISA Kit
1-CSB-E14050h
  • EUR 794.00
  • EUR 5028.00
  • EUR 2667.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163(sCD163) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

CD163 sgRNA CRISPR Lentivector set (Human)
K0405601 3 x 1.0 ug
EUR 339

Canine CD163 ELISA Kit
ELI-05600d 96 Tests
EUR 928

CD163 Antibody, HRP conjugated
1-CSB-PA801238LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

CD163 Antibody, FITC conjugated
1-CSB-PA801238LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

CD163 Antibody, Biotin conjugated
1-CSB-PA801238LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Cd163 Antibody, HRP conjugated
1-CSB-PA650452LB01MO
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

Cd163 Antibody, FITC conjugated
1-CSB-PA650452LC01MO
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

Cd163 Antibody, Biotin conjugated
1-CSB-PA650452LD01MO
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA

CD163(M130/1240) Antibody
BNC041240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC041240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL

Mouse CD163 shRNA Plasmid
20-abx979200
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

CD163(M130/1240) Antibody
BNC431240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC431240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCB1240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCB1240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC551240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC551240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC941240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC941240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC401240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC401240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC471240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC471240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCAP1240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCAP1240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC801240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC801240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC811240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC811240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC681240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC681240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC701240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC701240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC881240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC881240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC611240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC611240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCA1240-250 250uL
EUR 383
Description: Primary antibody against CD163(M130/1240), APC conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNUB1240-100 100uL
EUR 209
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL

CD163(M130/1240) Antibody
BNUB1240-500 500uL
EUR 458
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL

CD163(M130/1240) Antibody
BNUM1240-50 50uL
EUR 395
Description: Primary antibody against CD163(M130/1240), 1mg/mL

CD163(M130/1240) Antibody
BNCH1240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCH1240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC051240-100 100uL
EUR 199
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC051240-500 500uL
EUR 544
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCP1240-250 250uL
EUR 383
Description: Primary antibody against CD163(M130/1240), PerCP conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCR1240-250 250uL
EUR 383
Description: Primary antibody against CD163(M130/1240), RPE conjugate, Concentration: 0.1mg/mL

CD163 Monoclonal Antibody [RMC238A]
A68443 100 µl
EUR 628.55
Description: Ask the seller for details

CD163 Porcine Recombinant Protein
PROTQ2VL90 Regular: 50ug
EUR 317
Description: CD163 Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 805 amino acids and having a molecular mass of 87kDa.;The CD163 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques.

CD163 Protein Vector (Human) (pPB-C-His)
PV068177 500 ng
EUR 811

CD163 Protein Vector (Human) (pPB-N-His)
PV068178 500 ng
EUR 811

CD163 Protein Vector (Human) (pPM-C-HA)
PV068179 500 ng
EUR 811

CD163 Protein Vector (Human) (pPM-C-His)
PV068180 500 ng
EUR 811

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-10x96wellstestplate 10x96-wells test plate
EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-1x48wellstestplate 1x48-wells test plate
EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-1x96wellstestplate 1x96-wells test plate
EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-5x96wellstestplate 5x96-wells test plate
EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
4-SEB726Hu
  • EUR 4553.00
  • EUR 2405.00
  • EUR 613.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cluster Of Differentiation (CD163) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

CD163 sgRNA CRISPR Lentivector (Human) (Target 1)
K0405602 1.0 ug DNA
EUR 154

CD163 sgRNA CRISPR Lentivector (Human) (Target 2)
K0405603 1.0 ug DNA
EUR 154

CD163 sgRNA CRISPR Lentivector (Human) (Target 3)
K0405604 1.0 ug DNA
EUR 154

Recombinant Human CD163 Protein, His, Insect-1ug
QP11304-1ug 1ug
EUR 155

Recombinant Human CD163 Protein, His, Insect-50ug
QP11304-50ug 50ug
EUR 1261

Recombinant Human CD163 Protein, His, Insect-5ug
QP11304-5ug 5ug
EUR 201

Human Cluster Of Differentiation (CD163) CLIA Kit
20-abx493022
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Cluster of Differentiation 163 (CD163) Protein
20-abx652954
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Cluster Of Differentiation ELISA Kit (CD163)
RK01061 96 Tests
EUR 521

CD163 ELISA Kit (Human) : 96 Wells (OKAG00207)
OKAG00207 96 Wells
EUR 596
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 157 pg/mL

CD163 ELISA Kit (Human) : 96 Wells (OKEH02675)
OKEH02675 96 Wells
EUR 544
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.1 ng/mL

CD163 MonoSpecific Antibody, Unconjugated-20ug
9332-MSM1-P0 20ug
EUR 233

CD163 MonoSpecific Antibody, Unconjugated-100ug
9332-MSM1-P1 100ug
EUR 428

Recombinant Cluster Of Differentiation (CD163)
4-RPB726Mu01
  • EUR 556.96
  • EUR 252.00
  • EUR 1813.60
  • EUR 671.20
  • EUR 1242.40
  • EUR 436.00
  • EUR 4384.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Cluster Of Differentiation expressed in: E.coli

Recombinant Cluster Of Differentiation (CD163)
4-RPB726Ra01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Cluster Of Differentiation expressed in: E.coli

Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors

Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.

Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.

Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.

A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation

 

Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.

Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.

When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.

IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.

How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.

Leave a Reply

Your email address will not be published.