Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion
Clem
May 20, 2021
Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana
Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.
The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.
Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.
The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.
KEY POINTS:
• The TBSV-based transient expression system has been significantly improved.
- The necrotic effects caused by viral P19 protein were avoided by the usage of N. excelsiana as a host plant.
- The expression level of the non-fusion vector was similar to the most effective virus vectors reported so far.

grdr
Anti-CD163 |
DB-045-RTU-15 |
DB Biotech |
15 ml |
EUR 424.8 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB-045-RTU-7 |
DB Biotech |
7 ml |
EUR 277.2 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB045RTU-15 |
DB Biotech |
15 ml |
EUR 424.8 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB045RTU-7 |
DB Biotech |
7 ml |
EUR 277.2 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Mu-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Mu-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Ra-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Ra-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 418.8 |
Anti-CD163 Antibody |
A00812-1 |
BosterBio |
100ug/vial |
EUR 400.8 |
Anti-CD163 Antibody |
PA1874 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-CD163 Antibody |
PA1874-1 |
BosterBio |
100ug/vial |
EUR 400.8 |
Anti-CD163 antibody |
STJ110681 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
Anti-CD163 antibody |
STJ190009 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1) |
human CD163 antigen,CD163 LISA kit |
201-12-0247 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human CD163 antigen(CD163)ISA kit |
GA-E0263HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human CD163 antigen(CD163)ISA kit |
GA-E0263HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Anti-Hu CD163 Purified |
11-645-C025 |
ExBio |
0.025 mg |
EUR 118.8 |
Anti-Hu CD163 Purified |
11-645-C100 |
ExBio |
0.1 mg |
EUR 189.6 |
Anti-CD163/M130 Antibody |
A00812 |
BosterBio |
100ul |
EUR 476.4 |
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details. |
Anti-Hu CD163 APC |
1A-645-T025 |
ExBio |
25 tests |
EUR 168 |
Anti-Hu CD163 APC |
1A-645-T100 |
ExBio |
100 tests |
EUR 288 |
Anti-Hu CD163 PE |
1P-645-T025 |
ExBio |
25 tests |
EUR 168 |
Anti-Hu CD163 PE |
1P-645-T100 |
ExBio |
100 tests |
EUR 288 |
anti- CD163/M130 Antibody |
FNab09771 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against CD163/M130 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 336 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 336 |
Anti-CD163 Rabbit Monoclonal Antibody |
M00812 |
BosterBio |
100ug/vial |
EUR 476.4 |
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human. |
Anti-Hu CD163 PE-Cy7 |
T7-645-T025 |
ExBio |
25 tests |
EUR 184.8 |
Anti-Hu CD163 PE-Cy7 |
T7-645-T100 |
ExBio |
100 tests |
EUR 321.6 |
Anti-Hu CD163 PerCP-Cy5.5 |
T9-645-T025 |
ExBio |
25 tests |
EUR 235.2 |
Anti-Hu CD163 PerCP-Cy5.5 |
T9-645-T100 |
ExBio |
100 tests |
EUR 422.4 |
Purified Anti-human CD163 Antibody *GHI/61* |
11630000 |
AAT Bioquest |
100 ug |
EUR 365 |
APC Anti-human CD163 Antibody *GHI/61* |
116301B0 |
AAT Bioquest |
25 tests |
EUR 383 |
APC Anti-human CD163 Antibody *GHI/61* |
116301B1 |
AAT Bioquest |
100 tests |
EUR 995 |
APC Anti-human CD163 Antibody *GHI/61* |
116301B2 |
AAT Bioquest |
500 tests |
EUR 2984 |
FITC Anti-human CD163 Antibody *GHI/61* |
116301G0 |
AAT Bioquest |
100 tests |
EUR 547 |
FITC Anti-human CD163 Antibody *GHI/61* |
116301G1 |
AAT Bioquest |
500 tests |
EUR 2051 |
TRITC Anti-human CD163 Antibody *GHI/61* |
116301H0 |
AAT Bioquest |
100 tests |
EUR 547 |
TRITC Anti-human CD163 Antibody *GHI/61* |
116301H1 |
AAT Bioquest |
500 tests |
EUR 2051 |
PacBlue Anti-human CD163 Antibody *GHI/61* |
116301I0 |
AAT Bioquest |
100 tests |
EUR 547 |
PacBlue Anti-human CD163 Antibody *GHI/61* |
116301I1 |
AAT Bioquest |
500 tests |
EUR 2051 |
PacOrange Anti-human CD163 Antibody *GHI/61* |
116301J0 |
AAT Bioquest |
100 tests |
EUR 547 |
PacOrange Anti-human CD163 Antibody *GHI/61* |
116301J1 |
AAT Bioquest |
500 tests |
EUR 2051 |
PE Anti-human CD163 Antibody *GHI/61* |
116301K0 |
AAT Bioquest |
25 tests |
EUR 383 |
PE Anti-human CD163 Antibody *GHI/61* |
116301K1 |
AAT Bioquest |
100 tests |
EUR 995 |
PE Anti-human CD163 Antibody *GHI/61* |
116301K2 |
AAT Bioquest |
500 tests |
EUR 2984 |
PerCP Anti-human CD163 Antibody *GHI/61* |
116301S0 |
AAT Bioquest |
25 tests |
EUR 383 |
PerCP Anti-human CD163 Antibody *GHI/61* |
116301S1 |
AAT Bioquest |
100 tests |
EUR 995 |
PerCP Anti-human CD163 Antibody *GHI/61* |
116301S2 |
AAT Bioquest |
500 tests |
EUR 2984 |
Porcine CD163 antigen,CD163 LISA kit |
GA-E0045PC-48T |
GenAsia Biotech |
48T |
EUR 436.8 |
Porcine CD163 antigen,CD163 LISA kit |
GA-E0045PC-96T |
GenAsia Biotech |
96T |
EUR 708 |
Canine CD163 antigen(CD163 LISA kit |
GA-E0062CN-48T |
GenAsia Biotech |
48T |
EUR 482.4 |
Canine CD163 antigen(CD163 LISA kit |
GA-E0062CN-96T |
GenAsia Biotech |
96T |
EUR 820.8 |
CD163 Antibody |
49553-100ul |
SAB |
100ul |
EUR 399.6 |
CD163 Antibody |
49553-50ul |
SAB |
50ul |
EUR 286.8 |
CD163 Antibody |
37157-100ul |
SAB |
100ul |
EUR 302.4 |
CD163 antibody |
10R-6496 |
Fitzgerald |
100 ug |
EUR 243.6 |
Description: Mouse monoclonal CD163 antibody |
CD163 antibody |
10R-CD163aRT |
Fitzgerald |
250 ug |
EUR 859.2 |
Description: Mouse monoclonal CD163 antibody |
CD163 Antibody |
20-abx242956 |
Abbexa |
|
|
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CD163 siRNA |
20-abx910930 |
Abbexa |
|
|
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CD163 siRNA |
20-abx910931 |
Abbexa |
|
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CD163 Antibody |
1-CSB-PA801238LA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200 |
Cd163 Antibody |
1-CSB-PA650452LA01MO |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000 |
CD163 Antibody |
1-CSB-PA969205 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
Human CD163 shRNA Plasmid |
20-abx956176 |
Abbexa |
|
|
|
Human CD163 ELISA Kit |
LF-EK50890 |
Abfrontier |
1×96T |
EUR 777.6 |
Anti-Human CD163 DyLight® 488 conjugated Antibody |
A00812-Dyl488 |
BosterBio |
100ug/vial |
EUR 412.8 |
Anti-Human CD163 DyLight® 550 conjugated Antibody |
A00812-Dyl550 |
BosterBio |
100ug/vial |
EUR 412.8 |
Mouse Anti-Human CD163 monoclonal antibody, clone JID274 |
CABT-L2938-100uL500uL |
Creative Diagnostics |
100 uL, 500 uL |
EUR 602.4 |
APC/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301C0 |
AAT Bioquest |
25 tests |
EUR 429 |
APC/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301C1 |
AAT Bioquest |
100 tests |
EUR 1117 |
APC/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301C2 |
AAT Bioquest |
500 tests |
EUR 3351 |
PE/Cy5 Anti-human CD163 Antibody *GHI/61* |
116301L0 |
AAT Bioquest |
25 tests |
EUR 429 |
PE/Cy5 Anti-human CD163 Antibody *GHI/61* |
116301L1 |
AAT Bioquest |
100 tests |
EUR 1117 |
PE/Cy5 Anti-human CD163 Antibody *GHI/61* |
116301L2 |
AAT Bioquest |
500 tests |
EUR 3351 |
PE/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301M0 |
AAT Bioquest |
25 tests |
EUR 429 |
PE/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301M1 |
AAT Bioquest |
100 tests |
EUR 1117 |
PE/Cy7 Anti-human CD163 Antibody *GHI/61* |
116301M2 |
AAT Bioquest |
500 tests |
EUR 3351 |
PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61* |
116301T0 |
AAT Bioquest |
25 tests |
EUR 429 |
PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61* |
116301T1 |
AAT Bioquest |
100 tests |
EUR 1117 |
PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61* |
116301T2 |
AAT Bioquest |
500 tests |
EUR 3351 |
Human CellExp? CD163, Human recombinant |
9226-10 |
Biovision |
|
EUR 366 |
Human CellExp? CD163, Human recombinant |
9226-50 |
Biovision |
|
EUR 927.6 |
iFluor® 350 Anti-human CD163 Antibody *GHI/61* |
11630010 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 350 Anti-human CD163 Antibody *GHI/61* |
11630011 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 405 Anti-human CD163 Antibody *GHI/61* |
11630020 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 405 Anti-human CD163 Antibody *GHI/61* |
11630021 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 430 Anti-human CD163 Antibody *GHI/61* |
11630030 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 430 Anti-human CD163 Antibody *GHI/61* |
11630031 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 450 Anti-human CD163 Antibody *GHI/61* |
11630040 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 450 Anti-human CD163 Antibody *GHI/61* |
11630041 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 488 Anti-human CD163 Antibody *GHI/61* |
11630050 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 488 Anti-human CD163 Antibody *GHI/61* |
11630051 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 514 Anti-human CD163 Antibody *GHI/61* |
11630060 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 514 Anti-human CD163 Antibody *GHI/61* |
11630061 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 532 Anti-human CD163 Antibody *GHI/61* |
11630070 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 532 Anti-human CD163 Antibody *GHI/61* |
11630071 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 546 Anti-human CD163 Antibody *GHI/61* |
11630080 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 546 Anti-human CD163 Antibody *GHI/61* |
11630081 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 555 Anti-human CD163 Antibody *GHI/61* |
11630090 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 555 Anti-human CD163 Antibody *GHI/61* |
11630091 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 560 Anti-human CD163 Antibody *GHI/61* |
116300A0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 560 Anti-human CD163 Antibody *GHI/61* |
116300A1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 568 Anti-human CD163 Antibody *GHI/61* |
116300B0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 568 Anti-human CD163 Antibody *GHI/61* |
116300B1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 594 Anti-human CD163 Antibody *GHI/61* |
116300C0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 594 Anti-human CD163 Antibody *GHI/61* |
116300C1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 610 Anti-human CD163 Antibody *GHI/61* |
116300D0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 610 Anti-human CD163 Antibody *GHI/61* |
116300D1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 633 Anti-human CD163 Antibody *GHI/61* |
116300E0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 633 Anti-human CD163 Antibody *GHI/61* |
116300E1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 647 Anti-human CD163 Antibody *GHI/61* |
116300F0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 647 Anti-human CD163 Antibody *GHI/61* |
116300F1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 660 Anti-human CD163 Antibody *GHI/61* |
116300G0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 660 Anti-human CD163 Antibody *GHI/61* |
116300G1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 670 Anti-human CD163 Antibody *GHI/61* |
116300H0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 670 Anti-human CD163 Antibody *GHI/61* |
116300H1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 680 Anti-human CD163 Antibody *GHI/61* |
116300I0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 680 Anti-human CD163 Antibody *GHI/61* |
116300I1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 700 Anti-human CD163 Antibody *GHI/61* |
116300J0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 700 Anti-human CD163 Antibody *GHI/61* |
116300J1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 710 Anti-human CD163 Antibody *GHI/61* |
116300K0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 710 Anti-human CD163 Antibody *GHI/61* |
116300K1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 750 Anti-human CD163 Antibody *GHI/61* |
116300L0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 750 Anti-human CD163 Antibody *GHI/61* |
116300L1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 790 Anti-human CD163 Antibody *GHI/61* |
116300M0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 790 Anti-human CD163 Antibody *GHI/61* |
116300M1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 800 Anti-human CD163 Antibody *GHI/61* |
116300N0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 800 Anti-human CD163 Antibody *GHI/61* |
116300N1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 810 Anti-human CD163 Antibody *GHI/61* |
116300O0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 810 Anti-human CD163 Antibody *GHI/61* |
116300O1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 820 Anti-human CD163 Antibody *GHI/61* |
116300P0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 820 Anti-human CD163 Antibody *GHI/61* |
116300P1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 840 Anti-human CD163 Antibody *GHI/61* |
116300Q0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 840 Anti-human CD163 Antibody *GHI/61* |
116300Q1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® 860 Anti-human CD163 Antibody *GHI/61* |
116300R0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® 860 Anti-human CD163 Antibody *GHI/61* |
116300R1 |
AAT Bioquest |
500 tests |
EUR 2051 |
iFluor® A7 Anti-human CD163 Antibody *GHI/61* |
116300S0 |
AAT Bioquest |
100 tests |
EUR 547 |
iFluor® A7 Anti-human CD163 Antibody *GHI/61* |
116300S1 |
AAT Bioquest |
500 tests |
EUR 2051 |
mFluor™ UV375 Anti-human CD163 Antibody *GHI/61* |
116300X0 |
AAT Bioquest |
100 tests |
EUR 547 |
mFluor™ UV375 Anti-human CD163 Antibody *GHI/61* |
116300X1 |
AAT Bioquest |
500 tests |
EUR 2051 |
mFluor™ UV460 Anti-human CD163 Antibody *GHI/61* |
116300Y0 |
AAT Bioquest |
100 tests |
EUR 547 |
mFluor™ UV460 Anti-human CD163 Antibody *GHI/61* |
116300Y1 |
AAT Bioquest |
500 tests |
EUR 2051 |
Mouse Anti Rat Cd163 Monoclonal Antibody,Biotin |
DMABT-47168MR |
Creative Diagnostics |
0.1 mg |
EUR 1057.2 |
Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 |
M00812-1 |
BosterBio |
100uL |
EUR 462 |
Description: Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 tested in WB, IHC, reactive to Human |
CD163 ELISA Kit (Human) (OKBB00786) |
OKBB00786 |
Aviva Systems Biology |
96 Wells |
EUR 606 |
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml |
CD163 ELISA Kit (Human) (OKBB01513) |
OKBB01513 |
Aviva Systems Biology |
96 Wells |
EUR 684 |
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml |
Human CD163 PicoKine ELISA Kit |
EK0715 |
BosterBio |
96 wells |
EUR 510 |
Description: For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin). |
CD163 ELISA Kit (Human) (OKAN04666) |
OKAN04666 |
Aviva Systems Biology |
96 Wells |
EUR 950.4 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL |
CD163 ORF Vector (Human) (pORF) |
ORF017045 |
ABM |
1.0 ug DNA |
EUR 486 |
ELISA kit for Human CD163 |
EK5296 |
SAB |
96 tests |
EUR 663.6 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human CD163 in samples from serum, plasma, tissue homogenates and other biological fluids. |
QuickDetect? CD163 (Human) ELISA Kit |
K4422-100 |
Biovision |
|
EUR 966 |
CD163 ELISA Kit (Human) (OKCD07621) |
OKCD07621 |
Aviva Systems Biology |
96 Wells |
EUR 1123.2 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL |
CD163 antibody (PE) |
61R-1319 |
Fitzgerald |
100 tests |
EUR 483.6 |
Description: Mouse monoclonal CD163 antibody (PE) |
CD163 antibody (biotin) |
61R-1569 |
Fitzgerald |
100 ug |
EUR 418.8 |
Description: Mouse monoclonal CD163 antibody (biotin) |
CD163 antibody (biotin) |
61R-CD163BHUBT |
Fitzgerald |
100 ug |
EUR 1105.2 |
Description: Mouse monoclonal CD163 antibody (biotin) |
CD163 antibody (biotin) |
61R-CD163CHUBT |
Fitzgerald |
250 ug |
EUR 937.2 |
Description: Mouse monoclonal CD163 antibody (biotin) |
Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors
Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.
Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.
Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.
A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation
Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.
Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.
When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.
IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.
How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.