Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion
Clem
May 20, 2021
Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana
Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.
The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.
Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.
The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.
KEY POINTS:
• The TBSV-based transient expression system has been significantly improved.
- The necrotic effects caused by viral P19 protein were avoided by the usage of N. excelsiana as a host plant.
- The expression level of the non-fusion vector was similar to the most effective virus vectors reported so far.

grdr
Anti-CD163 |
DB-045-RTU-15 |
DB Biotech |
15 ml |
EUR 354 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB-045-RTU-7 |
DB Biotech |
7 ml |
EUR 231 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB045RTU-15 |
DB Biotech |
15 ml |
EUR 354 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
DB045RTU-7 |
DB Biotech |
7 ml |
EUR 231 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Mu-48T |
DL Develop |
48T |
EUR 508 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Mu-96T |
DL Develop |
96T |
EUR 661 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Ra-48T |
DL Develop |
48T |
EUR 508 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
DLR-CD163-Ra-96T |
DL Develop |
96T |
EUR 661 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RD-CD163-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 534 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 742 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 534 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
RDR-CD163-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 742 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
Anti-CD163 Antibody |
A00812-1 |
BosterBio |
100ug/vial |
EUR 334 |
Anti-CD163 antibody |
STJ190009 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1) |
Anti-CD163 antibody |
STJ110681 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
Anti-CD163 Antibody |
PA1874 |
BosterBio |
100ug/vial |
EUR 294 |
Anti-CD163 Antibody |
PA1874-1 |
BosterBio |
100ug/vial |
EUR 334 |
Anti-CD163/M130 Antibody |
A00812 |
BosterBio |
100ul |
EUR 397 |
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details. |
Anti-Hu CD163 Purified |
11-645-C025 |
ExBio |
0.025 mg |
EUR 99 |
Anti-Hu CD163 Purified |
11-645-C100 |
ExBio |
0.1 mg |
EUR 158 |
Anti-Hu CD163 APC |
1A-645-T025 |
ExBio |
25 tests |
EUR 140 |
Anti-Hu CD163 APC |
1A-645-T100 |
ExBio |
100 tests |
EUR 240 |
Anti-Hu CD163 PE |
1P-645-T025 |
ExBio |
25 tests |
EUR 140 |
Anti-Hu CD163 PE |
1P-645-T100 |
ExBio |
100 tests |
EUR 240 |
anti- CD163/M130 Antibody |
FNab09771 |
FN Test |
100µg |
EUR 548.75 |
|
Description: Antibody raised against CD163/M130 |
human CD163 antigen,CD163 LISA kit |
201-12-0247 |
SunredBio |
96 tests |
EUR 440 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human CD163 antigen(CD163)ISA kit |
GA-E0263HM-48T |
GenAsia Biotech |
48T |
EUR 289 |
Human CD163 antigen(CD163)ISA kit |
GA-E0263HM-96T |
GenAsia Biotech |
96T |
EUR 466 |
Polyclonal Goat anti-GST α-form |
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST μ-form |
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
Polyclonal Goat anti-GST p-form |
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
Anti-Hu CD163 PE-Cy7 |
T7-645-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD163 PE-Cy7 |
T7-645-T100 |
ExBio |
100 tests |
EUR 268 |
Anti-Hu CD163 PerCP-Cy5.5 |
T9-645-T025 |
ExBio |
25 tests |
EUR 196 |
Anti-Hu CD163 PerCP-Cy5.5 |
T9-645-T100 |
ExBio |
100 tests |
EUR 352 |
Anti-CD163 Rabbit Monoclonal Antibody |
M00812 |
BosterBio |
100ug/vial |
EUR 397 |
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human. |
Porcine CD163 antigen,CD163 LISA kit |
GA-E0045PC-48T |
GenAsia Biotech |
48T |
EUR 364 |
Porcine CD163 antigen,CD163 LISA kit |
GA-E0045PC-96T |
GenAsia Biotech |
96T |
EUR 590 |
Canine CD163 antigen(CD163 LISA kit |
GA-E0062CN-48T |
GenAsia Biotech |
48T |
EUR 402 |
Canine CD163 antigen(CD163 LISA kit |
GA-E0062CN-96T |
GenAsia Biotech |
96T |
EUR 684 |
CD163 antibody |
10R-CD163aRT |
Fitzgerald |
250 ug |
EUR 716 |
Description: Mouse monoclonal CD163 antibody |
CD163 antibody |
10R-6496 |
Fitzgerald |
100 ug |
EUR 203 |
Description: Mouse monoclonal CD163 antibody |
CD163 Antibody |
DF8235 |
Affbiotech |
200ul |
EUR 304 |
Description: CD163 Antibody detects endogenous levels of total CD163. |
CD163 Antibody |
1-CSB-PA969205 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
CD163 Antibody |
1-CSB-PA801238LA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200 |
Cd163 Antibody |
1-CSB-PA650452LA01MO |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000 |
CD163 siRNA |
20-abx910930 |
Abbexa |
|
|
|
CD163 siRNA |
20-abx910931 |
Abbexa |
|
|
|
CD163 Antibody |
20-abx242956 |
Abbexa |
|
|
|
CD163 Antibody |
37157-100ul |
SAB |
100ul |
EUR 252 |
CD163 Antibody |
49553-100ul |
SAB |
100ul |
EUR 333 |
CD163 Antibody |
49553-50ul |
SAB |
50ul |
EUR 239 |
Human CD163 ELISA Kit |
LF-EK50890 |
Abfrontier |
1×96T |
EUR 648 |
Human CD163 shRNA Plasmid |
20-abx956176 |
Abbexa |
|
|
|
Anti-Human CD163 DyLight® 488 conjugated Antibody |
A00812-Dyl488 |
BosterBio |
100ug/vial |
EUR 344 |
Anti-Human CD163 DyLight® 550 conjugated Antibody |
A00812-Dyl550 |
BosterBio |
100ug/vial |
EUR 344 |
Mouse Anti-Human CD163 monoclonal antibody, clone JID274 |
CABT-L2938-100uL500uL |
Creative Diagnostics |
100 uL, 500 uL |
EUR 502 |
Human CellExp? CD163, Human recombinant |
9226-10 |
Biovision |
|
EUR 305 |
Human CellExp? CD163, Human recombinant |
9226-50 |
Biovision |
|
EUR 773 |
Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 |
M00812-1 |
BosterBio |
100uL |
EUR 385 |
Description: Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 tested in WB, IHC, reactive to Human |
CD163 antibody (PE) |
61R-1319 |
Fitzgerald |
100 tests |
EUR 403 |
Description: Mouse monoclonal CD163 antibody (PE) |
CD163 antibody (biotin) |
61R-1569 |
Fitzgerald |
100 ug |
EUR 349 |
Description: Mouse monoclonal CD163 antibody (biotin) |
CD163 antibody (biotin) |
61R-CD163BHUBT |
Fitzgerald |
100 ug |
EUR 921 |
Description: Mouse monoclonal CD163 antibody (biotin) |
CD163 antibody (biotin) |
61R-CD163CHUBT |
Fitzgerald |
250 ug |
EUR 781 |
Description: Mouse monoclonal CD163 antibody (biotin) |
CD163 Blocking Peptide |
DF8235-BP |
Affbiotech |
1mg |
EUR 195 |
Porcine CD163 Protein |
20-abx260472 |
Abbexa |
-
EUR 230.00
-
EUR 2332.00
-
EUR 328.00
|
|
|
Cd163 Polyclonal Antibody |
A53832 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: Ask the seller for details |
CD163 Rabbit pAb |
A8383-100ul |
Abclonal |
100 ul |
EUR 308 |
CD163 Rabbit pAb |
A8383-200ul |
Abclonal |
200 ul |
EUR 459 |
CD163 Rabbit pAb |
A8383-20ul |
Abclonal |
20 ul |
EUR 183 |
CD163 Rabbit pAb |
A8383-50ul |
Abclonal |
50 ul |
EUR 223 |
CD163 Conjugated Antibody |
C49553 |
SAB |
100ul |
EUR 397 |
CD163 Conjugated Antibody |
C37157 |
SAB |
100ul |
EUR 397 |
CD163 ORF Vector (Human) (pORF) |
ORF017045 |
ABM |
1.0 ug DNA |
EUR 405 |
QuickDetect? CD163 (Human) ELISA Kit |
K4422-100 |
Biovision |
|
EUR 805 |
Human CD163 PicoKine ELISA Kit |
EK0715 |
BosterBio |
96 wells |
EUR 425 |
Description: For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin). |
ELISA kit for Human CD163 |
EK5296 |
SAB |
96 tests |
EUR 553 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human CD163 in samples from serum, plasma, tissue homogenates and other biological fluids. |
CD163 ELISA Kit (Human) (OKCD07621) |
OKCD07621 |
Aviva Systems Biology |
96 Wells |
EUR 936 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL |
CD163 ELISA Kit (Human) (OKAN04666) |
OKAN04666 |
Aviva Systems Biology |
96 Wells |
EUR 792 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL |
CD163 ELISA Kit (Human) (OKBB00786) |
OKBB00786 |
Aviva Systems Biology |
96 Wells |
EUR 505 |
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml |
CD163 ELISA Kit (Human) (OKBB01513) |
OKBB01513 |
Aviva Systems Biology |
96 Wells |
EUR 570 |
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml |
Human soluble CD163(sCD163) ELISA Kit |
CSB-E14050h-24T |
Cusabio |
1 plate of 24 wells |
EUR 165 |
|
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163 (sCD163) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human soluble CD163(sCD163) ELISA Kit |
1-CSB-E14050h |
Cusabio |
-
EUR 794.00
-
EUR 5028.00
-
EUR 2667.00
|
-
1 plate of 96 wells
-
10 plates of 96 wells each
-
5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163(sCD163) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
CD163 sgRNA CRISPR Lentivector set (Human) |
K0405601 |
ABM |
3 x 1.0 ug |
EUR 339 |
CD163 Antibody, HRP conjugated |
1-CSB-PA801238LB01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
CD163 Antibody, FITC conjugated |
1-CSB-PA801238LC01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
CD163 Antibody, Biotin conjugated |
1-CSB-PA801238LD01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Cd163 Antibody, HRP conjugated |
1-CSB-PA650452LB01MO |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA |
Cd163 Antibody, FITC conjugated |
1-CSB-PA650452LC01MO |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA |
Cd163 Antibody, Biotin conjugated |
1-CSB-PA650452LD01MO |
Cusabio |
|
|
|
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA |
CD163(M130/1240) Antibody |
BNC041240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC041240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL |
Mouse CD163 shRNA Plasmid |
20-abx979200 |
Abbexa |
|
|
|
CD163(M130/1240) Antibody |
BNC431240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC431240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCB1240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCB1240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC551240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC551240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC941240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC941240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC401240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC401240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC471240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC471240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCAP1240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCAP1240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC801240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC801240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC811240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC811240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC681240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC681240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC701240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC701240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC881240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC881240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC611240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC611240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCA1240-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against CD163(M130/1240), APC conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNUB1240-100 |
Biotium |
100uL |
EUR 209 |
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL |
CD163(M130/1240) Antibody |
BNUB1240-500 |
Biotium |
500uL |
EUR 458 |
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL |
CD163(M130/1240) Antibody |
BNUM1240-50 |
Biotium |
50uL |
EUR 395 |
Description: Primary antibody against CD163(M130/1240), 1mg/mL |
CD163(M130/1240) Antibody |
BNCH1240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCH1240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC051240-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNC051240-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCP1240-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against CD163(M130/1240), PerCP conjugate, Concentration: 0.1mg/mL |
CD163(M130/1240) Antibody |
BNCR1240-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against CD163(M130/1240), RPE conjugate, Concentration: 0.1mg/mL |
CD163 Monoclonal Antibody [RMC238A] |
A68443 |
EpiGentek |
100 µl |
EUR 628.55 |
Description: Ask the seller for details |
CD163 Porcine Recombinant Protein |
PROTQ2VL90 |
BosterBio |
Regular: 50ug |
EUR 317 |
Description: CD163 Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 805 amino acids and having a molecular mass of 87kDa.;The CD163 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques. |
CD163 Protein Vector (Human) (pPB-C-His) |
PV068177 |
ABM |
500 ng |
EUR 811 |
CD163 Protein Vector (Human) (pPB-N-His) |
PV068178 |
ABM |
500 ng |
EUR 811 |
CD163 Protein Vector (Human) (pPM-C-HA) |
PV068179 |
ABM |
500 ng |
EUR 811 |
CD163 Protein Vector (Human) (pPM-C-His) |
PV068180 |
ABM |
500 ng |
EUR 811 |
Human Cluster Of Differentiation (CD163) ELISA Kit |
SEB726Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4502.43 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids. |
Human Cluster Of Differentiation (CD163) ELISA Kit |
SEB726Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 458.44 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids. |
Human Cluster Of Differentiation (CD163) ELISA Kit |
SEB726Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 612.05 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids. |
Human Cluster Of Differentiation (CD163) ELISA Kit |
SEB726Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2454.23 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids. |
Human Cluster Of Differentiation (CD163) ELISA Kit |
4-SEB726Hu |
Cloud-Clone |
-
EUR 4553.00
-
EUR 2405.00
-
EUR 613.00
|
-
10 plates of 96 wells
-
5 plates of 96 wells
-
1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cluster Of Differentiation (CD163) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
CD163 sgRNA CRISPR Lentivector (Human) (Target 1) |
K0405602 |
ABM |
1.0 ug DNA |
EUR 154 |
CD163 sgRNA CRISPR Lentivector (Human) (Target 2) |
K0405603 |
ABM |
1.0 ug DNA |
EUR 154 |
CD163 sgRNA CRISPR Lentivector (Human) (Target 3) |
K0405604 |
ABM |
1.0 ug DNA |
EUR 154 |
Recombinant Human CD163 Protein, His, Insect-1ug |
QP11304-1ug |
EnQuireBio |
1ug |
EUR 155 |
Recombinant Human CD163 Protein, His, Insect-50ug |
QP11304-50ug |
EnQuireBio |
50ug |
EUR 1261 |
Recombinant Human CD163 Protein, His, Insect-5ug |
QP11304-5ug |
EnQuireBio |
5ug |
EUR 201 |
Human Cluster Of Differentiation (CD163) CLIA Kit |
20-abx493022 |
Abbexa |
-
EUR 7973.00
-
EUR 4246.00
-
EUR 981.00
|
-
10 × 96 tests
-
5 × 96 tests
-
96 tests
|
|
Human Cluster of Differentiation 163 (CD163) Protein |
20-abx652954 |
Abbexa |
-
EUR 578.00
-
EUR 258.00
-
EUR 1720.00
-
EUR 690.00
-
EUR 425.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
ELISA kit for Human Soluble CD163 (sCD163) |
KTE62428-48T |
Abbkine |
48T |
EUR 332 |
|
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Soluble CD163 (sCD163) |
KTE62428-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2115 |
|
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Soluble CD163 (sCD163) |
KTE62428-96T |
Abbkine |
96T |
EUR 539 |
|
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Human Cluster Of Differentiation ELISA Kit (CD163) |
RK01061 |
Abclonal |
96 Tests |
EUR 521 |
CD163 ELISA Kit (Human) : 96 Wells (OKAG00207) |
OKAG00207 |
Aviva Systems Biology |
96 Wells |
EUR 596 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 157 pg/mL |
CD163 ELISA Kit (Human) : 96 Wells (OKEH02675) |
OKEH02675 |
Aviva Systems Biology |
96 Wells |
EUR 544 |
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.1 ng/mL |
CD163 MonoSpecific Antibody, Unconjugated-20ug |
9332-MSM1-P0 |
EnQuireBio |
20ug |
EUR 233 |
CD163 MonoSpecific Antibody, Unconjugated-100ug |
9332-MSM1-P1 |
EnQuireBio |
100ug |
EUR 428 |
Recombinant Cluster Of Differentiation (CD163) |
4-RPB726Mu01 |
Cloud-Clone |
-
EUR 556.96
-
EUR 252.00
-
EUR 1813.60
-
EUR 671.20
-
EUR 1242.40
-
EUR 436.00
-
EUR 4384.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Mouse Cluster Of Differentiation expressed in: E.coli |
Recombinant Cluster Of Differentiation (CD163) |
4-RPB726Ra01 |
Cloud-Clone |
-
EUR 530.08
-
EUR 245.00
-
EUR 1712.80
-
EUR 637.60
-
EUR 1175.20
-
EUR 418.00
-
EUR 4132.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Rat Cluster Of Differentiation expressed in: E.coli |
Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors
Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.
Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.
Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.
A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation
Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.
Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.
When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.
IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.
How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.