Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion

Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana 

Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.

The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.

Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.

The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.

 

KEY POINTS:

• The TBSV-based transient expression system has been significantly improved.

• The necrotic effects caused by viral P19 protein were avoided by the usage of N.   excelsiana as a host plant.

• The expression level of the non-fusion vector was similar to the most effective virus   vectors reported so far.

Anti-CD163
DB-045-RTU-15	DB Biotech	15 ml	EUR 354
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB-045-RTU-7	DB Biotech	7 ml	EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB045RTU-15	DB Biotech	15 ml	EUR 354
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-CD163
DB045RTU-7	DB Biotech	7 ml	EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Mu-48Tests	Reddot Biotech	48 Tests	EUR 511

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709

Rat Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Ra-48Tests	Reddot Biotech	48 Tests	EUR 511

Rat Cluster Of Differentiation (CD163) ELISA Kit
RD-CD163-Ra-96Tests	Reddot Biotech	96 Tests	EUR 709

Mouse Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Mu-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids.

Mouse Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Mu-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids.

Rat Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Ra-48T	DL Develop	48T	EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Cluster Of Differentiation (CD163) ELISA Kit
DLR-CD163-Ra-96T	DL Develop	96T	EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Mu-48Tests	Reddot Biotech	48 Tests	EUR 534

Mouse Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Mu-96Tests	Reddot Biotech	96 Tests	EUR 742

Rat Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Cluster Of Differentiation (CD163) ELISA Kit
RDR-CD163-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

Anti-CD163 Antibody
PA1874	BosterBio	100ug/vial	EUR 294

Anti-CD163 Antibody
PA1874-1	BosterBio	100ug/vial	EUR 334

Anti-CD163 antibody
STJ160082	St John's Laboratory	1 mL C	EUR 941

Anti-CD163 antibody
STJ16100302	St John's Laboratory	100 µg	EUR 720

Anti-CD163 antibody
STJ16100324	St John's Laboratory	1 mL	EUR 979

Anti-CD163 antibody
STJ180301	St John's Laboratory	0.1 ml	EUR 215

Anti-CD163 antibody
STJ190009	St John's Laboratory	200 µl	EUR 197
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1)

Anti-CD163 Antibody
STJ500423	St John's Laboratory	100 µg	EUR 476

Anti-CD163 Antibody
STJ500424	St John's Laboratory	100 µg	EUR 476

Anti-CD163 antibody
STJ110681	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Anti-CD163 Antibody
A00812-1	BosterBio	100ug/vial	EUR 334

Anti-CD163 Antibody BIOTIN
STJ500425	St John's Laboratory	100 µg	EUR 586

Anti-CD163 Antibody FITC
STJ500426	St John's Laboratory	100 µg	EUR 586

Anti-CD163 Antibody BIOTIN
STJ500427	St John's Laboratory	100 µg	EUR 586

Anti-CD163 Antibody FITC
STJ500428	St John's Laboratory	100 µg	EUR 586

Anti-CD163/M130 Antibody
A00812	BosterBio	100ul	EUR 397
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details.

Anti-Hu CD163 Purified
11-645-C025	ExBio	0.025 mg	EUR 99

Anti-Hu CD163 Purified
11-645-C100	ExBio	0.1 mg	EUR 158

Anti-Hu CD163 APC
1A-645-T025	ExBio	25 tests	EUR 140

Anti-Hu CD163 APC
1A-645-T100	ExBio	100 tests	EUR 240

Anti-Hu CD163 PE
1P-645-T025	ExBio	25 tests	EUR 140

Anti-Hu CD163 PE
1P-645-T100	ExBio	100 tests	EUR 240

anti- CD163/M130 Antibody
FNab09771	FN Test	100µg	EUR 548.75
Description: Antibody raised against CD163/M130

Anti-CD163/M130 antibody
PAab09771	Lifescience Market	100 ug	EUR 355

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-48T	GenAsia Biotech	48T	EUR 289

Human CD163 antigen(CD163)ISA kit
GA-E0263HM-96T	GenAsia Biotech	96T	EUR 466

human CD163 antigen,CD163 LISA kit
201-12-0247	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human CD163 antigen(CD163)ISA kit
QY-E05066	Qayee Biotechnology	96T	EUR 361

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47162MH	Creative Diagnostics	25 µg	EUR 372

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47163MH	Creative Diagnostics	0.2 mg	EUR 881

Anti-CD163 Rabbit Monoclonal Antibody
M00812	BosterBio	100ug/vial	EUR 397
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human.

Anti-Hu CD163 PE-Cy7
T7-645-T025	ExBio	25 tests	EUR 154

Anti-Hu CD163 PE-Cy7
T7-645-T100	ExBio	100 tests	EUR 268

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T025	ExBio	25 tests	EUR 196

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T100	ExBio	100 tests	EUR 352

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-48T	GenAsia Biotech	48T	EUR 364

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-96T	GenAsia Biotech	96T	EUR 590

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-48T	GenAsia Biotech	48T	EUR 402

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-96T	GenAsia Biotech	96T	EUR 684

CD163 Antibody
1-CSB-PA969205	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

CD163 Antibody
1-CSB-PA801238LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

Cd163 Antibody
1-CSB-PA650452LA01MO	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

CD163 Antibody
37157-100ul	SAB	100ul	EUR 252

CD163 siRNA
20-abx910930	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

CD163 siRNA
20-abx910931	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

CD163 Antibody
20-abx242956	Abbexa	EUR 467.00 EUR 328.00	100 ul 50 ul

CD163 Antibody
49553-100ul	SAB	100ul	EUR 333

CD163 Antibody
49553-50ul	SAB	50ul	EUR 239

CD163 Antibody
ABD8235	Lifescience Market	100 ug	EUR 438

CD163 Antibody
DF8235	Affbiotech	200ul	EUR 304
Description: CD163 Antibody detects endogenous levels of total CD163.

CD163 antibody
10R-6496	Fitzgerald	100 ug	EUR 203
Description: Mouse monoclonal CD163 antibody

CD163 antibody
10R-CD163aRT	Fitzgerald	250 ug	EUR 716
Description: Mouse monoclonal CD163 antibody

Mouse Anti Rat Cd163 Monoclonal Antibody
DMABT-47170MR	Creative Diagnostics	0.25 mg	EUR 741

Mouse Anti Rat Cd163 Monoclonal Antibody
DMABT-47171MR	Creative Diagnostics	0.1 mg	EUR 559

Anti-Human CD163 DyLight® 488 conjugated Antibody
A00812-Dyl488	BosterBio	100ug/vial	EUR 344

Anti-Human CD163 DyLight® 550 conjugated Antibody
A00812-Dyl550	BosterBio	100ug/vial	EUR 344

Mouse Anti-Human CD163 monoclonal antibody, clone JID274
CABT-L2938-100uL500uL	Creative Diagnostics	100 uL, 500 uL	EUR 502

Human CD163 shRNA Plasmid
20-abx956176	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human CD163 ELISA Kit
ELA-E1726h	Lifescience Market	96 Tests	EUR 824

Human CD163 ELISA Kit
LF-EK50890	Abfrontier	1×96T	EUR 648

Human CellExp? CD163, Human recombinant
9226-10	Biovision		EUR 305

Human CellExp? CD163, Human recombinant
9226-50	Biovision		EUR 773

Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
M00812-1	BosterBio	100uL	EUR 385
Description: Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 tested in WB, IHC, reactive to Human

Mouse Anti Rat Cd163 Monoclonal Antibody,Biotin
DMABT-47168MR	Creative Diagnostics	0.1 mg	EUR 881

Mouse Anti Rat Cd163 Monoclonal Antibody,FITC
DMABT-47169MR	Creative Diagnostics	0.1 mg	EUR 881

Mouse Anti Rat Cd163 Monoclonal Antibody,RPE
DMABT-47172MR	Creative Diagnostics	100 TEST	EUR 892

Mouse Anti Pig Cd163 Monoclonal Antibody,FITC
CABT-47164MP	Creative Diagnostics	0.1 mg	EUR 881

Mouse Anti Pig Cd163 Monoclonal Antibody,RPE
CABT-47166MP	Creative Diagnostics	100 TEST	EUR 715

Recombinant Porcine CD163
7-04750	CHI Scientific	10µg	Ask for price

Recombinant Porcine CD163
7-04751	CHI Scientific	50µg	Ask for price

Recombinant Porcine CD163
7-04752	CHI Scientific	1mg	Ask for price

Cd163 Polyclonal Antibody
A53832	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

CD163 Rabbit pAb
A8383-100ul	Abclonal	100 ul	EUR 308

CD163 Rabbit pAb
A8383-200ul	Abclonal	200 ul	EUR 459

CD163 Rabbit pAb
A8383-20ul	Abclonal	20 ul	EUR 183

CD163 Rabbit pAb
A8383-50ul	Abclonal	50 ul	EUR 223

Porcine CD163 Protein
20-abx260472	Abbexa	EUR 230.00 EUR 2332.00 EUR 328.00	10 ug 1 mg 50 ug

CD163 Conjugated Antibody
C37157	SAB	100ul	EUR 397

CD163 Conjugated Antibody
C49553	SAB	100ul	EUR 397

CD163 Blocking Peptide
DF8235-BP	Affbiotech	1mg	EUR 195

CD163 antibody (PE)
61R-1319	Fitzgerald	100 tests	EUR 403
Description: Mouse monoclonal CD163 antibody (PE)

CD163 antibody (biotin)
61R-1569	Fitzgerald	100 ug	EUR 349
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 antibody (biotin)
61R-CD163BHUBT	Fitzgerald	100 ug	EUR 921
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 antibody (biotin)
61R-CD163CHUBT	Fitzgerald	250 ug	EUR 781
Description: Mouse monoclonal CD163 antibody (biotin)

CD163 Recombinant Protein
96-843	ProSci	0.1 mg	EUR 595.25
Description: Scavenger receptor cysteine-rich type 1 protein M130 (CD163) is also known as hemoglobin scavenger receptor, which is a scavenger receptor for the hemoglobin-haptoglobin complex. CD163 has also been shown to mark cells of monocyte/macrophage lineage. A soluble form of the receptor exists in plasma, commonly denoted sCD163. sCD163 is generated by ectodomain shedding of the membrane bound receptor. sCD163 is upregulated in a large range of inflammatory diseases including liver cirrhosis, type 2 diabetes, macrophage activation syndrome, Gaucher's disease, sepsis, HIV infection, rheumatoid arthritis and Hodgkin Lymphoma.

Human CD163 PicoKine ELISA Kit
EK0715	BosterBio	96 wells	EUR 425
Description: For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin).

CD163 ELISA Kit (Human) (OKCD07621)
OKCD07621	Aviva Systems Biology	96 Wells	EUR 936
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL

CD163 ELISA Kit (Human) (OKAN04666)
OKAN04666	Aviva Systems Biology	96 Wells	EUR 792
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL

CD163 ELISA Kit (Human) (OKBB00786)
OKBB00786	Aviva Systems Biology	96 Wells	EUR 505
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

CD163 ELISA Kit (Human) (OKBB01513)
OKBB01513	Aviva Systems Biology	96 Wells	EUR 570
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

ELISA kit for Human CD163
EK5296	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human CD163 in samples from serum, plasma, tissue homogenates and other biological fluids.

CD163 ORF Vector (Human) (pORF)
ORF017045	ABM	1.0 ug DNA	EUR 405

QuickDetect? CD163 (Human) ELISA Kit
K4422-100	Biovision		EUR 805

Human soluble CD163(sCD163) ELISA Kit
CSB-E14050h-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163 (sCD163) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human soluble CD163(sCD163) ELISA Kit
1-CSB-E14050h	Cusabio	EUR 794.00 EUR 5028.00 EUR 2667.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human soluble CD163(sCD163) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

CD163 sgRNA CRISPR Lentivector set (Human)
K0405601	ABM	3 x 1.0 ug	EUR 339

CD163 Antibody, HRP conjugated
1-CSB-PA801238LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

CD163 Antibody, FITC conjugated
1-CSB-PA801238LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

CD163 Antibody, Biotin conjugated
1-CSB-PA801238LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Cd163 Antibody, HRP conjugated
1-CSB-PA650452LB01MO	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

Cd163 Antibody, FITC conjugated
1-CSB-PA650452LC01MO	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

Cd163 Antibody, Biotin conjugated
1-CSB-PA650452LD01MO	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA

CD163 Porcine Recombinant Protein
PROTQ2VL90	BosterBio	Regular: 50ug	EUR 317
Description: CD163 Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 805 amino acids and having a molecular mass of 87kDa.;The CD163 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques.

CD163(M130/1240) Antibody
BNC041240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC041240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF405S conjugate, Concentration: 0.1mg/mL

Mouse CD163 shRNA Plasmid
20-abx979200	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

CD163(M130/1240) Antibody
BNC471240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC471240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF647 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCAP1240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCAP1240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC801240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC801240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF680 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC811240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC811240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF680R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC681240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC681240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF568 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC701240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC701240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF770 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC881240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC881240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF488A conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC611240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC611240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF660R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCA1240-250	Biotium	250uL	EUR 383
Description: Primary antibody against CD163(M130/1240), APC conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNUB1240-100	Biotium	100uL	EUR 209
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL

CD163(M130/1240) Antibody
BNUB1240-500	Biotium	500uL	EUR 458
Description: Primary antibody against CD163(M130/1240), Concentration: 0.2mg/mL

CD163(M130/1240) Antibody
BNUM1240-50	Biotium	50uL	EUR 395
Description: Primary antibody against CD163(M130/1240), 1mg/mL

CD163(M130/1240) Antibody
BNCH1240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCH1240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC051240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC051240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF405M conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCP1240-250	Biotium	250uL	EUR 383
Description: Primary antibody against CD163(M130/1240), PerCP conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCR1240-250	Biotium	250uL	EUR 383
Description: Primary antibody against CD163(M130/1240), RPE conjugate, Concentration: 0.1mg/mL

CD163 Monoclonal Antibody [RMC238A]
A68443	EpiGentek	100 µl	EUR 628.55
Description: Ask the seller for details

CD163(M130/1240) Antibody
BNC431240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC431240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF543 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCB1240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNCB1240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), Biotin conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC551240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC551240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF555 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC941240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC941240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF594 conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC401240-100	Biotium	100uL	EUR 199
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL

CD163(M130/1240) Antibody
BNC401240-500	Biotium	500uL	EUR 544
Description: Primary antibody against CD163(M130/1240), CF640R conjugate, Concentration: 0.1mg/mL

Canine CD163 ELISA Kit
ELI-05600d	Lifescience Market	96 Tests	EUR 928

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
SEB726Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cluster Of Differentiation (CD163) in serum, plasma, tissue homogenates and other biological fluids.

Human Cluster Of Differentiation (CD163) ELISA Kit
4-SEB726Hu	Cloud-Clone	EUR 4553.00 EUR 2405.00 EUR 613.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cluster Of Differentiation (CD163) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-48T	Abbkine	48T	EUR 332
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Soluble CD163 (sCD163)
KTE62428-96T	Abbkine	96T	EUR 539
Description: Quantitative sandwich ELISA for measuring Human Soluble CD163 (sCD163) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Cluster Of Differentiation (CD163) CLIA Kit
20-abx493022	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Human Cluster of Differentiation 163 (CD163) Protein
20-abx652954	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Cluster Of Differentiation ELISA Kit (CD163)
RK01061	Abclonal	96 Tests	EUR 521

CD163 ELISA Kit (Human) : 96 Wells (OKEH02675)
OKEH02675	Aviva Systems Biology	96 Wells	EUR 544
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.1 ng/mL

CD163 ELISA Kit (Human) : 96 Wells (OKAG00207)
OKAG00207	Aviva Systems Biology	96 Wells	EUR 596
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 157 pg/mL

CD163 sgRNA CRISPR Lentivector (Human) (Target 1)
K0405602	ABM	1.0 ug DNA	EUR 154

CD163 sgRNA CRISPR Lentivector (Human) (Target 2)
K0405603	ABM	1.0 ug DNA	EUR 154

CD163 sgRNA CRISPR Lentivector (Human) (Target 3)
K0405604	ABM	1.0 ug DNA	EUR 154

CD163 Protein Vector (Human) (pPB-C-His)
PV068177	ABM	500 ng	EUR 811

CD163 Protein Vector (Human) (pPB-N-His)
PV068178	ABM	500 ng	EUR 811

CD163 Protein Vector (Human) (pPM-C-HA)
PV068179	ABM	500 ng	EUR 811

CD163 Protein Vector (Human) (pPM-C-His)
PV068180	ABM	500 ng	EUR 811

Recombinant Human CD163 Protein, His, Insect-1ug
QP11304-1ug	EnQuireBio	1ug	EUR 155

Recombinant Human CD163 Protein, His, Insect-50ug
QP11304-50ug	EnQuireBio	50ug	EUR 1261

Recombinant Human CD163 Protein, His, Insect-5ug
QP11304-5ug	EnQuireBio	5ug	EUR 201

Recombinant Cluster Of Differentiation (CD163)
4-RPB726Mu01	Cloud-Clone	EUR 556.96 EUR 252.00 EUR 1813.60 EUR 671.20 EUR 1242.40 EUR 436.00 EUR 4384.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Cluster Of Differentiation expressed in: E.coli

Recombinant Cluster Of Differentiation (CD163)
4-RPB726Ra01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Cluster Of Differentiation expressed in: E.coli

Polyclonal CD163 Antibody (N-Terminus)
APR02509G	Leading Biology	10.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CD163 (N-Terminus). This antibody is tested and proven to work in the following applications:

Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors

Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.

Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.

Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.

A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation

 

Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.

Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.

When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.

IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.

How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.