Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion

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Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana 

Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors.

The genome of Tomato bushy stunt virus (TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants.

Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant, Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly used Nicotiana benthamiana host associated with expression of TBSV-encoded P19 protein.

The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene in N. excelsiana leaves, demonstrating that N. excelsiana is an ideal host plant for TBSV-mediated heterologous gene expression. Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.

 

KEY POINTS:

• The TBSV-based transient expression system has been significantly improved.

  • The necrotic effects caused by viral P19 protein were avoided by the usage of N.   excelsiana as a host plant.
  • The expression level of the non-fusion vector was similar to the most effective virus   vectors reported so far.
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PE anti-human CD163

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PE anti-human CD163

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Description: Available in various conjugation types.

Anti-Human CD163 Antibody

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EUR 399
Description: CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes.

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Description: Available in various conjugation types.

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Description: Available in various conjugation types.

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Description: Available in various conjugation types.

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EUR 1191.67
Description: Available in various conjugation types.

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Description: Available in various conjugation types.

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Description: Rabbit Anti Human CD163 Monoclonal Clone ABCC-3

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Description: Available in various conjugation types.

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Description: Available in various conjugation types.

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Recombinant Human CD163 Protein

RP02295 500μg
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116301K0 25 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301K0-25tests 25 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*

116301K1 100 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301K1-100tests 100 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE Anti-human CD163 Antibody *GHI/61*

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Human CellExp? CD163, Human recombinant

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Human CellExp? CD163, Human recombinant

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APC Anti-human CD163 Antibody *GHI/61*

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*

116301B0-25tests 25 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*

116301B1 100 tests
EUR 1015
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*

116301B1-100tests 100 tests
EUR 995
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC Anti-human CD163 Antibody *GHI/61*

116301B2 500 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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Description: Available in various conjugation types.

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Description: Available in various conjugation types.

FITC Anti-human CD163 Antibody *GHI/61*

116301G0 100 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

FITC Anti-human CD163 Antibody *GHI/61*

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EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301G1 500 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301G1-500tests 500 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301H0-100tests 100 tests
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

TRITC Anti-human CD163 Antibody *GHI/61*

116301H1 500 tests
EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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116301H1-500tests 500 tests
EUR 2051
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

116301S0 25 tests
EUR 391
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

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EUR 383
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

116301S1 100 tests
EUR 1015
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

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EUR 995
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

116301S2 500 tests
EUR 3044
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP Anti-human CD163 Antibody *GHI/61*

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EUR 2984
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*

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EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*

116301I0-100tests 100 tests
EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacBlue Anti-human CD163 Antibody *GHI/61*

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Purified Anti-human CD163 Antibody *GHI/61*

11630000 100 ug
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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*

116301J0 100 tests
EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*

116301J0-100tests 100 tests
EUR 547
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*

116301J1 500 tests
EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PacOrange Anti-human CD163 Antibody *GHI/61*

116301J1-500tests 500 tests
EUR 2051
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

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TA320238 100 µg Ask for price

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CD3-H52E8 1mg
EUR 374.5
Description: Human CD163, His Tag (CD3-H52E8) is expressed from human 293 cells (HEK293). It contains AA Ser 42 - Ser 1045 (Accession # Q86VB7).

Human CD163 PicoKine ELISA Kit

EK0715 96 wells
EUR 510
Description: For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin).

Human CD163 knockout cell line

ABC-KH2671 1 vial Ask for price
Description: Human CD163 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

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ABC-KD2671 1 vial Ask for price
Description: Human CD163 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CD163 Protein Lysate 20ug

IHUCD163PLLY20UG each
EUR 213
Description: Human CD163 Protein Lysate 20ug

Mouse Recombinant anti-Human CD163 Recombinant Antibody

xAP-0136 100ug
EUR 280

CD163 ORF Vector (Human) (pORF)

ORF017045 1.0 ug DNA
EUR 486

Canine CD163 antigen(CD163 LISA kit

GA-E0062CN-48T 48T
EUR 482.4

Canine CD163 antigen(CD163 LISA kit

GA-E0062CN-96T 96T
EUR 820.8

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EUR 436.8

Porcine CD163 antigen,CD163 LISA kit

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Porcine CD163 antigen, CD163 LISA kit

KLP0040 1 x 96 wells
EUR 341

Porcine CD163 antigen,CD163 LISA kit

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Porcine CD163 antigen,CD163 LISA kit

YLA0139PO-96T 96T Ask for price

Rat CD163 antigen, CD163 GENLISA ELISA

KLR2081 1 x 96 wells
EUR 341

Cd163 (untagged) - Mouse CD163 antigen (Cd163), transcript variant 2, (10ug)

MC223654 10 µg Ask for price

Cd163 (untagged) - Mouse CD163 antigen (Cd163), transcript variant 1, (10ug)

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CD163 ELISA Kit (Human) (OKCD07621)

OKCD07621 96 Wells
EUR 1123.2
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.273ng/mL

CD163 ELISA Kit (Human) (OKAN04666)

OKAN04666 96 Wells
EUR 950.4
Description: Description of target: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.273 ng/mL

CD163 ELISA Kit (Human) (OKBB00786)

OKBB00786 96 Wells
EUR 606
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

CD163 ELISA Kit (Human) (OKBB01513)

OKBB01513 96 Wells
EUR 684
Description: Description of target: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <150pg/ml

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L0 25 tests
EUR 438
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L0-25tests 25 tests
EUR 429
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L1 100 tests
EUR 1140
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L1-100tests 100 tests
EUR 1117
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L2 500 tests
EUR 3419
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy5 Anti-human CD163 Antibody *GHI/61*

116301L2-500tests 500 tests
EUR 3351
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M0 25 tests
EUR 438
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M0-25tests 25 tests
EUR 429
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M1 100 tests
EUR 1140
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M1-100tests 100 tests
EUR 1117
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M2 500 tests
EUR 3419
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PE/Cy7 Anti-human CD163 Antibody *GHI/61*

116301M2-500tests 500 tests
EUR 3351
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

QuickDetect? CD163 (Human) ELISA Kit

K4422-100 each
EUR 966

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C0 25 tests
EUR 438
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C0-25tests 25 tests
EUR 429
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C1 100 tests
EUR 1140
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C1-100tests 100 tests
EUR 1117
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C2 500 tests
EUR 3419
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

APC/Cy7 Anti-human CD163 Antibody *GHI/61*

116301C2-500tests 500 tests
EUR 3351
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Lenti ORF particles, CD163 (mGFP-tagged) - Human CD163 molecule (CD163), transcript variant 1, 200ul, >10^7 TU/mL

RC208557L2V 200 µl Ask for price

Lenti ORF particles, CD163 (mGFP-tagged) - Human CD163 molecule (CD163), transcript variant 1, 200ul, >10^7 TU/mL

RC208557L4V 200 µl Ask for price

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T0 25 tests
EUR 438
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T0-25tests 25 tests
EUR 429
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T1 100 tests
EUR 1140
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T1-100tests 100 tests
EUR 1117
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T2 500 tests
EUR 3419
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

PerCP/Cy5.5 Anti-human CD163 Antibody *GHI/61*

116301T2-500tests 500 tests
EUR 3351
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Anti-Hu CD163 PE-Cy™7

T7-645-T025 25 tests
EUR 137.5

Anti-Hu CD163 PE-Cy™7

T7-645-T100 100 tests
EUR 275

mFluorâ„¢ UV375 Anti-human CD163 Antibody *GHI/61*

116300X0 100 tests
EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

mFluorâ„¢ UV375 Anti-human CD163 Antibody *GHI/61*

116300X1 500 tests
EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

mFluorâ„¢ UV460 Anti-human CD163 Antibody *GHI/61*

116300Y0 100 tests
EUR 558
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

mFluorâ„¢ UV460 Anti-human CD163 Antibody *GHI/61*

116300Y1 500 tests
EUR 2093
Description: GHI/61 is an anti-human monoclonal antibody that forms an immune complex with the CD163 antigen.

Anti-Hu CD163 PerCP-Cy™5.5

T9-645-T025 25 tests
EUR 165

Anti-Hu CD163 PerCP-Cy™5.5

T9-645-T100 100 tests
EUR 330

PerCP/Cyanine5.5 Anti-Human CD163 Antibody[GHI/61]

E-AB-F1298J-100Tests 100Tests
EUR 300
Description: FCM

PerCP/Cyanine5.5 Anti-Human CD163 Antibody[GHI/61]

E-AB-F1298J-100Tests2 100Tests×2
EUR 450
Description: FCM

PerCP/Cyanine5.5 Anti-Human CD163 Antibody[GHI/61]

E-AB-F1298J-20Tests 20Tests
EUR 120
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295E-100Tests 100Tests
EUR 145
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295E-100Tests2 100Tests×2
EUR 220
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295E-50Tests 50Tests
EUR 85
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295UE-100ug 100ug
EUR 360
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295UE-25ug 25ug
EUR 100
Description: FCM

APC Anti-Mouse CD163 Antibody[S15049F]

E-AB-F1295UE-each each Ask for price
Description: FCM

CD163 antibody

10R-CD163aRT 250 ug Ask for price
Description: Mouse monoclonal CD163 antibody

CD163 antibody

10R-6496 100 ug
EUR 156
Description: Mouse monoclonal CD163 antibody

CD163 Antibody

37157 100ul
EUR 319

CD163 Antibody

37157-100ul 100ul
EUR 302.4

CD163 Antibody

49553 100ul
EUR 499

CD163 Antibody

49553-100ul 100ul
EUR 399.6

CD163 Antibody

49553-50ul 50ul
EUR 286.8

CD163 Antibody

20-abx242956
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  • 100 ul
  • 50 ul

CD163 Antibody

1-CSB-PA801238LA01HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

CD163 Antibody

1-CSB-PA969205
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  • 100ul
  • 50ul
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

CD163 Antibody

E037157 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CD163 Antibody

E19-8235-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

CD163 Antibody

E19-8235-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

CD163 Antibody

DF8235 200ul
EUR 420

CD163 Antibody

DF8235-100ul 100ul
EUR 280

CD163 Antibody

DF8235-200ul 200ul
EUR 350

CD163 Antibody

E38PA9656 100ul
EUR 225
Description: Available in various conjugation types.

CD163 Antibody

E39-10799 100ug/100ul
EUR 225
Description: Available in various conjugation types.

CD163 Antibody

ABD8235 100 ug
EUR 525.6

Cd163 Antibody

1-CSB-PA650452LA01MO
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

CD163 Antibody

GWB-C1B36B 0.025 mg Ask for price

CD163 Antibody

GWB-BFAF4C 0.2 mg Ask for price

CD163 Antibody

F50316-0.08ML 0.08 ml
EUR 140.25
Description: CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

CD163 Antibody

F50316-0.4ML 0.4 ml
EUR 322.15
Description: CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

CD163 Antibody

R20390-0.1ML 100ul
EUR 409

CD163 Antibody

R30973 100 ug
EUR 356.15
Description: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptor for the hemoglobin-haptoglobincomplex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al.(2000) mapped the CD163 gene to chromosome 12p13.3. Specific CD163-mediated endocytosis of haptoglobin-hemoglobin complexes was measurable in cells transfected with CD163 cDNA and in CD163-expressing myelomonocytic lymphoma cells.CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by anti-CD163 antibodies. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.

CD163 Antibody

R30974 100 ug
EUR 356.15
Description: Cluster of Differentiation 163, also known as Hemoglobin scavenger receptor, is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptorfor the hemoglobin-haptoglobin complex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al.(2000) mapped the gene to chromosome 12p13.3. CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by CD163 antibody. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.

CD163 Antibody

RQ4259 100 ug
EUR 356.15
Description: CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

CD163 Antibody

V8757-100UG 100 ug
EUR 349.3
Description: CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

CD163 Antibody

V8757-20UG 20 ug
EUR 153.3
Description: CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

CD163 Antibody

V8757SAF-100UG 100 ug
EUR 349.3
Description: CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

CD163 Antibody

V8227-100UG 100 ug
EUR 349.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody

V8227-20UG 20 ug
EUR 153.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody

V8227SAF-100UG 100 ug
EUR 349.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody

V8415-100UG 100 ug
EUR 424.15
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody

V8415-20UG 20 ug
EUR 186.15
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody

V8415SAF-100UG 100 ug
EUR 424.15
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

Effect of Serotype and Strain Diversity on Dengue Virus Replication in Australian Mosquito Vectors

Dengue virus (DENV) is the most important mosquito-borne viral pathogen of humans, comprising four serotypes (DENV-1 to -4) with a myriad of genotypes and strains. The kinetics of DENV replication within the mosquito following ingestion of a blood meal influence the pathogen’s ability to reach the salivary glands and thus the transmission potential. The influence of DENV serotype and strain diversity on virus kinetics in the two main vector species, Aedes aegypti and Ae. albopictus, has been poorly characterized. We tested whether DENV replication kinetics vary systematically among serotypes and strains, using Australian strains of the two vectors.

Mosquitoes were blood fed with two strains per serotype, and sampled at 3, 6, 10 and 14-days post-exposure. Virus infection in mosquito bodies, and dissemination of virus to legs and wings, was detected using qRT-PCR. For both vectors, we found significant differences among serotypes in proportions of mosquitoes infected, with higher numbers for DENV-1 and -2 versus other serotypes.

Consistent with this, we observed that DENV-1 and -2 generally replicated to higher RNA levels than other serotypes, particularly at earlier time points. There were no significant differences in either speed of infection or dissemination between the mosquito species. Our results suggest that DENV diversity may have important epidemiological consequences by influencing virus kinetics in mosquito vectors.

A Plant Virus Ensures Viral Stability in the Hemolymph of Vector Insects through Suppressing Prophenoloxidase Activation

 

Most plant viruses require vector insects for transmission. Viral stability in the hemolymph of vector insects is a prerequisite for successful transmission of persistent plant viruses. However, knowledge of whether the proteolytic activation of prophenoloxidase (PPO) affects the stability of persistent plant viruses remains elusive.

Here, we explored the interplay between rice stripe virus (RSV) and the PPO cascade of the vector small brown planthopper. Phenoloxidase (PO) activity was suppressed by RSV by approximately 60%.

When the PPO cascade was activated, we found distinct melanization around RSV particles and serious damage to viral stability in the hemolymph. Viral suppression of PO activity was derived from obstruction of proteolytic cleavage of PPOs by binding of the viral nonstructural protein NS3. These results indicate that RSV attenuates the PPO response to ensure viral stability in the hemolymph of vector insects. Our research provides enlightening cues for controlling the transmission of vector-borne viruses.

IMPORTANCE Large ratios of vector-borne plant viruses circulate in the hemolymph of their vector insects before entering the salivary glands to be transmitted to plants. The stability of virions in the hemolymph is vital in this process. Activation of the proteolytic prophenoloxidase (PPO) to produce active phenoloxidase (PO) is one of the major innate immune pathways in insect hemolymph.

How a plant virus copes with the PPO immune reaction in its vector insect remains unclear. Here, we report that the PPO affects the stability of rice stripe virus (RSV), a notorious rice virus, in the hemolymph of a vector insect, the small brown planthopper. RSV suppresses PPO activation using viral nonstructural protein. Once the level of PO activity is elevated, RSV is melanized and eliminated from the hemolymph. Our work gives valuable clues for developing novel strategies for controlling the transmission of vector-borne plant viruses.

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